Basb027 proteins and genes from moraxella catarrhalis, antigens, antibodies, and uses
AssigneeGlaxosmithkline Biologicals SA
InventorCarlota Vinals y de Bassols
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Abstract
The invention provides BASB027 polypeptides and polynucleotides encoding BASB027 polypeptides and methods for producing such polypeptides by recombinant techniques. Also provided are diagnostic, prophylactic and therapeutic uses.
Claims16
-13. (canceled)
An isolated polynucleotide encoding a polypeptide or the full complement to the isolated polynucleotide, wherein the polypeptide is selected from (a) a polypeptide comprising an amino acid sequence at least 90% identical to the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:4;
- (b) a polypeptide comprising an amino acid sequence at least 95% identical to the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:4;
- or (c) a polypeptide comprising an immunogenic fragment that is at least 15 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:2 or SEQ ID NO:4.
An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence hybridizes to the full complement of SEQ ID NO:1 or SEQ ID NO:3 minus the complement of any stop codon, wherein the hybridization conditions include incubation at 42° C. in a solution comprising 50% formamide, 5×SSC (150 mM NaCl, 15 mM trisodium citrate), 50 mM sodium phosphate (pH7.6), 5×Denhardt's solution, 10% dextran sulfate, and 20 micrograms/ml denatured, sheared salmon sperm DNA, followed by washing in 0.1×SSC at 65° C.;
- and, wherein the polynucleotide sequence is identical to SEQ ID NO:1 or SEQ ID NO:3 minus any terminal stop codon, except that, over the entire length corresponding to SEQ ID NOs:1 or 3 minus any terminal stop codon, nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn•y),
- wherein xn is the total number of nucleotides in SEQ ID NO:1 or SEQ ID NO:3 minus any terminal stop codon, y is at least 0.95, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- and wherein the polynucleotide sequence detects Moraxella catarrhalis.
An isolated polynucleotide segment comprising a polynucleotide sequence or the full complement of the entire length of the polynucleotide sequence, wherein the polynucleotide sequence is identical to SEQ ID NO:1 or SEQ ID NO:3 minus any terminal stop codon, except that, over the entire length corresponding to SEQ ID NO:1 or SEQ ID NO:3 minus any terminal stop codon nn nucleotides are substituted, inserted or deleted, wherein nn satisfies the following expression
- nn≦xn−(xn•y),
- wherein xn is the total number of nucleotides in SEQ ID NO:1 or SEQ ID NO:3 minus any terminal stop codon y is at least 0.90, and wherein any non-integer product of xn and y is rounded down to the nearest integer before subtracting the product from xn;
- and wherein the polynucleotide sequence detects Moraxella catarrhalis.
-33. (canceled)
Dependencies are inferred from the claim text.