Method for Preparing a Covalently Cross Linked Oligomer of Amyloid Beta Peptides
OwnerKaren M. Grimm, Joseph G. Joyce +2inventors
InventorsKaren M. Grimm, Joseph G. Joyce, Xiaoping Liang, Denise Nawrocki
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Abstract
The invention relates to a method for the preparation of a stabilized cross-linked oligomer of amyloid beta using a near-zero length bifunctional cross-linking agent for use as an immunogen for the generation of antibodies for the treatment of Alzheimer's Disease and other conditions related to abnormal amyloid beta aggregation. A preferred bifunctional cross-linking agent is 1,5-difluoro-2,4-dinitrobenzene (DFDNB).
Claims22
A method for producing a covalently stabilized antigen comprising:
- a. preparing a solution of solubilized Aβ-derived peptide;
- b. combining said solution with a near-zero length bifunctional cross-linking agent to form a mixture;
- c. incubating said mixture at a temperature for a period of time sufficient to effect cross-linking;
- and
- d. providing the cross-linked product of step (c) to be used as an antigen.
A method of producing a covalently stabilized antigen having a conformational epitope of Aβ comprising:
- a. preparing a solution of solubilized Aβ-derived peptide;
- b. combining said solution with a bifunctional cross-linking agent to form a mixture;
- c. incubating said mixture at a temperature for a period of time sufficient to effect cross-linking;
- and
- d. providing the cross-linked product of step (c) to be used as an antigen.
A method for producing a purified covalently stabilized antigen comprising:
- a. preparing a solution of solubilized Aβ-derived peptide;
- b. combining said solution with a bifunctional cross-linking agent to form a mixture;
- c. incubating said mixture at a temperature for a period of time sufficient to effect cross-linking;
- d. purifying the plurality of cross-linked products from step (c) using chromatographic separation based on mass of said cross-linked products;
- and
- e. providing the purified product of step (d) to be used as an antigen.
Dependencies are inferred from the claim text.