Reimmunization and antibody design
AssigneeCarviar APS
InventorJanus Beierholm Larsen
Life of this patent
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Abstract
The present invention relates to methods for harvesting of antibodies from an antibody library. The antibodies are harvested by utilising a certain epitope that is analogous to the epitope of the antigen used for immunization but that may differ in global physical and biochemical properties allowing the production of antibodies against antigens that normally can not be utilised as immunizing agents. The present invention furthermore relate to fields of use for harvested antigens in industry, agriculture and healthcare.
Claims101
A method for obtaining, from a plurality of different IgY antibodies specific for one or more primary antigens, one or more IgY antibodies, but not all of said plurality of IgY antibodies, wherein said isolated IgY antibodies are also specific for one or more secondary antigens, said method comprising the steps of
- i) generating a plurality of IgY antibodies by immunizing each of a plurality of avian organisms of the same or different species with a composition comprising a plurality of different first antigens, thereby generating a plurality of different IgY antibodies,
- ii) providing one or more secondary antigen(s) to be tested,
- iii) contacting said one or more secondary antigen(s) with the plurality of different IgY antibodies specific for one or more primary antigens as generated in step i), and
- iv) obtaining one or more IgY antibodies having a specificity for both one or more of said primary antigens and one or more of said secondary antigens,
- v) wherein the primary and secondary antigens are not identical, and
- vi) wherein the obtained IgY antibodies specific for said one or more secondary antigens have been raised against said one or more primary antigens only.
(canceled)
Use of a library of secondary antigens, such as peptides, or nucleic acid molecules encoding such peptides, in the generation of a library of antibodies having been raised by one or more primary antigens, wherein said primary and secondary antigens are not identical.
A method for obtaining, from a plurality of different IgY antibodies specific for one or more primary antigens, one or more IgY antibodies, but not all of said plurality of IgY antibodies, wherein said isolated IgY antibodies are also specific for one or more secondary antigens, said method comprising the steps of
- i) generating a plurality of IgY antibodies by immunizing each of a plurality of avian organisms of the same or different species with a composition comprising a plurality of different first antigens, thereby generating a plurality of different IgY antibodies,
- ii) providing one or more secondary antigen(s) to be tested,
- iii) contacting said one or more secondary antigen(s) with the plurality of different IgY antibodies specific for one or more primary antigens as generated in step i), and
- iv) obtaining one or more IgY antibodies having a specificity for both one or more of said primary antigens and one or more of said secondary antigens,
- v) wherein the primary and secondary antigens are identical, and
- vi) wherein the obtained IgY antibodies specific for said one or more secondary antigens have been raised against said one or more primary antigens only.
Dependencies are inferred from the claim text.