Image Forming Method and Microscope Device
AssigneeNikon Corporation
InventorsHisao Osawa, Yumiko Ouchi
Life of this patent
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Abstract
More images than spatial modulation components of a lighting light are acquired, and a least square method is applied to the imaged plurality of images to obtain signal components. Accordingly, a ultra-resolution microscope which uses a spatially-modified lighting light to illuminate a sample, arithmetic-processes acquired images to thereby demodulate a given spatial modulation, and obtains a high-resolution sample image, wherein a high-resolution image reduced in noise components can be obtained.
Claims7
An image forming method in which a sample is illuminated with spatially modulated illuminating light, light from the sample illuminated by the illuminating light is focused as an image, and a sample image is formed by image calculation processing from the acquired image, wherein
- a greater number of images than the number of spatially modulated components contained in the light emitted from the sample as a result of the illuminating light on the sample are acquired, and the most reasonable sample image is formed by applying the method of least squares with the amount by which the plurality of picked-up images are varied when these images are picked up for each of the spatial frequency components constituting the plurality of picked-up images taken as a parameter.
A microscope device comprising:
- a light source;
- an illumination optical system which illuminates a sample with light from the light source;
- a diffraction grating which is disposed in a position that is optically conjugate with the sample in the illumination optical system;
- a modulation device which modulates the diffraction grating, and which illuminates the sample with spatially modulated light;
- an image pickup means which picks up spatially modulated sample images from the sample;
- and
- an image forming device which forms the most reasonable sample image by applying the method of least squares with the amount by which the plurality of picked-up images are varied when these images are picked up for each of the spatial frequency components constituting a greater number of images than the number of spatially modulated components contained in the light emitted from the sample as a result of the illuminating light on the sample picked up by the image pickup means taken as a parameter.
Dependencies are inferred from the claim text.