Ras-mediated epigenetic silencing effectors and uses thereof
AssigneeUniversity of Massachusetts
InventorsMichael R. Green, Claude Gazin, Narendra Wajapeyee
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Abstract
The invention relates to methods for inhibiting gene silencing, methods for inhibiting cell proliferation, methods for inhibiting Ras mediated tumor growth, methods for screening for regulators of FAS expression, and methods for identifying inhibitors of Ras mediated tumor growth.
Claims63
A method for inhibiting gene silencing in a cell comprising reducing expression of one or more Ras epigenetic silencing effectors (RESEs) in the cell.
A method for inhibiting silencing of a gene in a cell comprising reducing the interaction of one or more Ras epigenetic silencing effectors (RESEs) with a regulatory DNA sequence of the gene in the cell.
A method for inhibiting proliferation of a cell comprising reducing expression of one or more Ras epigenetic silencing effectors (RESEs) in the cell.
A method for inhibiting RAS-mediated growth of a tumor comprising reducing expression of one or more Ras epigenetic silencing effectors (RESEs) in the tumor.
A method for screening for regulators of FAS expression comprising:
- (i) transducing eukaryotic cells with pools of a plurality of retroviruses, wherein individual retroviruses in the plurality comprise a nucleic acid encoding a product that modulates expression of at least one gene encoded in the genome of the transduced cells;
- (ii) isolating FAS positive transduced cells;
- and
- (iii) identifying the transduced nucleic acid.
A method for identifying compounds or compositions that inhibit RAS-mediated tumor growth comprising contacting a cell with a compound or composition and assaying for decreased expression of one or more Ras epigenetic silencing effectors (RESEs).
Dependencies are inferred from the claim text.