Method and Device for Analyzing Biomolecules With Track - Etched Polymeric Layers
AssigneesThe United States of America/NIH, 20/20 GeneSystems, Inc.
InventorsVladimir KNEZEVIC, Dan-Paul Hartmann, Michael R. Emmert-Buck
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Abstract
The present disclosure provides methods, devices and kits that permit large numbers of target biomolecules to be detected simultaneously in samples originating from a multi-sample holder, such as a multi-well plate. One specific example method is a method of making multiple substantial replicas of a biomolecular content of a multi-well sample holder. Devices and kits for carrying out the described methods are also provided.
Claims45
1-27. (canceled)
A method of analyzing biomolecules in a biological sample, comprising the steps:
- providing a stack of at least two track-etched polymeric layers;
- wherein one or more of the layers are coated with a capture probe having a specific affinity to a target molecule in the biological sample;
- transferring said biomolecules through said layers and capturing a portion of said biomolecules on one or more of said layers;
- and analyzing one or more of the captured biomolecules
Method of analyzing selected biomolecules in a sample comprising:
- passing the sample through a plurality of track-etched polycarbonate membranes coated with a capture molecule having an affinity to the selected biomolecule;
- capturing the selected biomolecule on the membranes;
- separating the membranes for analysis;
- analyzing the captured biomolecule.
Dependencies are inferred from the claim text.