GFABS: GFP-based biosensors possessing the binding properties of antibodies
AssigneeWisconsin Alumni Research Foundation
InventorsEric V. Shusta, Tej Pavoor
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Abstract
A family of GFP scaffolds capable of accommodating two proximal, randomized binding loops is disclosed. GFP-based binders binding with nanomolar affinity are developed from a library of these GFP scaffolds.
Claims22
A fluorescent biosensor comprising a fluorescent protein scaffold with heterologous amino acid sequence insertions between Glu172 and Asp173 and between Asp 102 and Asp 103, wherein the scaffold has the mutations D 19N, F64L, and A87T and wherein the protein is selected from the group of GFP and GFP variants.
An expression library comprising at least two fluorescent protein scaffolds, wherein the fluorescent protein scaffolds are not identical.
A fluorescent biosensor comprising a fluorescent protein scaffold with heterologous amino acid sequence insertions, wherein the scaffold has mutations corresponding to D19N, F64L and A87T, wherein the protein is selected from the group consisting of GFP and GFP variants.
Dependencies are inferred from the claim text.