Enhanced butanol producing microorganisms and method for preparing butanol using the same
AssigneeBiofuelchem Co.
InventorsSang Yup Lee, Jin Hwan Park, Eleftherios Terry Papoutsakis
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Abstract
The present invention relates to a recombinant mutant microorganism having enhanced butanol producing capacity and a method for producing butanol using the same. In the microorganism, genes coding for enzymes responsible for the biosynthesis of lactate, ethanol and/or acetate are deleted or attenuated and genes coding for enzymes involved in butanol biosynthesis are introduced and amplified.
Claims25
-21. (canceled)
A recombinant mutant microorganism having high butanol productivity, in which at least one selected from the group consisting of genes coding for enzymes involved in lactate biosynthesis, genes coding for enzymes involved in acetate biosynthesis, and genes coding for enzymes involved in ethanol biosynthesis is deleted or attenuated;
- and at least one gene coding for an enzyme involved in butanol biosynthesis is introduced or amplified.
A recombinant mutant microorganism having high butanol productivity, in which genes coding for enzymes involved in lactate biosynthesis, genes coding for enzymes involved in acetate biosynthesis, and genes coding for enzymes involved in ethanol biosynthesis are deleted or attenuated;
- and genes coding for thiolase (THL), 3-hydroxybutyryl-CoA dehydrogenase (BHBD), crotonase (CRO), butyryl-CoA dehydrogenase (BCD), butyraldehyde dehydrogenase (AAD), butanol dehydrogenase (BDH), a chaperone protein (groESL), and BCD co-factors (etfAB) are introduced or amplified.
Dependencies are inferred from the claim text.