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US 2010/0136638 A1Published application
Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase
AssigneeNovozymes A/S
InventorsHans H. Liao, Ravi R. Gokarn, Steven John Gort, Holly Jean Jessen, Olga V. Selifonova
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Abstract
Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed.
Claims38
A transformed prokaryotic cell comprising:
- an exogenous nucleic acid molecule encoding a beta-alanine/pyruvate aminotransferase having at least 95% sequence identity to SEQ ID NO:
- 18, wherein the beta-alanine/pyruvate aminotransferase is capable of producing malonate semialdehyde and alanine from beta-alanine and pyruvate, and
- an exogenous nucleic acid molecule encoding an alanine 2,3-aminomutase, wherein the alanine 2,3-aminomutase is capable of producing beta-alanine from alpha-alanine, wherein the prokaryotic cell produces 3-hydroxypropionic acid (3-HP) from beta-alanine.
Dependencies are inferred from the claim text.