Method for the high level expression of active lymphotoxin-beta receptor immunoglobulin chimeric proteins and their purification
AssigneeBiogen Idec MA Inc.
InventorsJeffrey BROWNING, Konrad MIATKOWSKI, Werner MEIER
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Abstract
Methods for high level expression of active lymphotoxin-β receptor immunoglobulin chimeric proteins and their purification.
Claims26
A method for the expression of high yields of active TNF receptor family member-Ig Fc fusion proteins and minimizing expression of inactive TNF receptor family member-Ig Fc fusion proteins comprising culturing a host cell transformed with DNA molecule encoding a desired TNF receptor-Ig fusion protein in a mammalian cell culture system having a temperature of less than or equal to 32° C.
A method for the expression of high yields of active TNF receptor family member-Ig Fc fusion proteins and minimizing expression of inactive TNF receptor family member-Ig Fc fusion proteins comprising culturing a host cell transformed with DNA molecule encoding a desired TNF receptor-Ig fusion protein in a mammalian cell culture system having a temperature of less than or equal to 35° C. and recovering the active TNF receptor family member-Ig Fc fusion proteins from the culture system.
A method for obtaining active LTβR-Ig Fc fusion proteins and minimizing expression of inactive LTβR-Ig Fc fusion proteins comprising culturing a mammalian host cell transformed with DNA molecule encoding a desired TNF receptor-Ig fusion protein in cell culture medium at a temperature of less than or equal to 30° C. and isolating the active LTβR-Ig Fc fusion proteins from the culture medium using hydrophobic interaction chromatography.
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