Probe, probe set, probe-immobilized carrier, and genetic testing method
AssigneeCanon Kabushiki Kaisha
InventorMasahiro Kawaguchi
Life of this patent
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Abstract
A nucleic acid probe for classification of pathogenic bacterial species is capable of collectively detecting bacterial strains of the same species and differentially detecting them from other bacterial species. Any one of the base sequences of SEQ ID NOS. 62 to 64 or a combination of at least two of them is used for detecting the gene of an infectious disease pathogenic bacterium.
Claims13
A probe for detecting a gene of infectious disease pathogenic bacterium, Enterococcus avium, capable of hybridizing with the 16S rRNA coding region of, Enterococcus avium, said probe showing a hybridization intensity weak enough to avoid cross-hybridization with the 16S rRNA coding region of Enterococcus faecalis, said probe having any one of the following base sequences (1) to (4):
- (1) TTTGAAAGGCGCTTTTGCGTCACTG (SEQ ID NO. 62) or a complementary sequence thereof;
- (2) CAAGGATGAGAGTAGAACGTTCATCCCTTG (SEQ ID NO. 63) or a complementary sequence thereof;
- (3) CAAGGATGAGAGTAGAATGTTCATCCCTTG (SEQ ID NO. 64) or a complementary sequence thereof;
- and
- (4) a modified sequence prepared such that any one of the sequences of SEQ ID NOS. 62 to 64 and the complementary sequences thereof is subjected to base deletion, substitution, or addition as far as the modified sequence retains a function as the probe.
A probe set for detecting a gene of infectious disease pathogenic bacterium, Enterococcus avium, including at least two probes selected from the following items (A) to (L):
- (A) a probe having a base sequence represented by
- TTTGAAAGGCGCTTTTGCGTCACTG;
- (SEQ ID NO. 62)
- (B) a probe having a base sequence represented by
- CAAGGATGAGAGTAGAACGTTCATCCCTTG;
- (SEQ ID NO. 63)
- (C) a probe having a base sequence represented by
- ATATACCGTCAAGCTTCCACAGCGA;
- (SEQ ID NO:
- 64)
- (D) a probe having a complementary sequence of the base sequence represented by SEQ ID NO. 62;
- (E) a probe having a complementary sequence of the base sequence represented by SEQ ID NO. 63;
- (F) a probe having a complementary sequence of the base sequence represented by SEQ ID NO. 64;
- (G) a probe having a modified sequence obtained by base deletion, substitution, or addition on the base sequence represented by SEQ ID NO. 62 as far as it retains the function of a probe for detecting the gene of Enterococcus avium;
- (H) a probe having a modified sequence obtained by base deletion, substitution, or addition on the base sequence represented by SEQ ID NO. 63 as far as it retains the function of a probe for detecting the gene of Enterococcus avium;
- (I) a probe having a modified sequence obtained by base deletion, substitution, or addition on the base sequence represented by SEQ ID NO. 64 as far as it retains the function of a probe for detecting the gene of Enterococcus avium;
- (J) a probe having a modified sequence obtained by base deletion, substitution, or addition on the complementary sequence of the base sequence represented by SEQ ID NO. 62 as far as it retains the function of a probe for detecting the gene of Enterococcus avium;
- (K) a probe having a modified sequence obtained by base deletion, substitution, or addition on the complementary sequence of the base sequence represented by SEQ ID NO. 63 as far as it retains the function of a probe for detecting the gene of Enterococcus avium;
- and
- (L) a probe having a modified sequence obtained by base deletion, substitution, or addition on the complementary sequence of the base sequence represented by SEQ ID NO. 64 as far as it retains the function of a probe for detecting the gene of Enterococcus avium.
Dependencies are inferred from the claim text.