Fiat nucleic acids and proteins and uses thereof
AssigneeSHRINERS HOSPITALS FOR CHILDREN, a Colorado corporation
InventorRene St-Arnaud
Life of this patent
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Abstract
The invention provides FIAT nucleic acids and proteins, and methods of using such nucleic acids and proteins.
Claims16
1-19. (canceled)
A method for identifying a candidate compound, the method comprising:
- (a) providing a first polypeptide that:
- (i) comprises a Factor Inhibiting ATF4-mediated Transcription (FIAT) polypeptide or a fragment thereof;
- and
- (ii) displays activating transcription factor-4 (ATF4)-binding ability;
- (b) providing a second polypeptide that:
- (i) comprises an ATF4 polypeptide or a fragment thereof;
- and
- (ii) displays FIAT binding ability;
- (c) contacting the first and second polypeptides in the presence of a test compound;
- and
- (d) comparing the level of binding between the first and second polypeptides in the presence of the test compound with the level of binding in the absence of the test compound, wherein a different level of binding in the presence of the test compound than in its absence indicates that the test compound is a candidate compound.
24-52. (canceled)
An isolated nucleic acid molecule comprising:
- (i) a first nucleic acid sequence encoding a fragment of a Factor Inhibiting ATF4-mediated Transcription (FIAT) polypeptide that displays activating transcription factor-4 (ATF4) protein binding ability;
- and
- (ii) a second non-FIAT nucleic acid sequence unrelated to the first nucleic acid sequence that is directly contiguous with the 3′ or 5′ end of the first nucleic acid sequence.
A cell comprising an exogenously-introduced nucleic acid molecule that comprises:
- (i) a first nucleic acid sequence encoding a fragment of a Factor Inhibiting ATF4-mediated Transcription (FIAT) polypeptide that displays activating transcription factor-4 (ATF4) protein binding ability;
- and
- (ii) a second non-FIAT nucleic acid sequence unrelated to the first nucleic acid sequence that is directly contiguous with the 3′ or 5′ end of the first nucleic acid sequence.
A recombinant protein comprising amino acids 184-231 of SEQ ID NO:2 directly linked at its C-terminal end, N-terminal end, or both C-terminal and N-terminal ends, to at least one non-Factor Inhibiting ATF4-mediated Transcription (FIAT) polypeptide amino acid sequence.
Dependencies are inferred from the claim text.