Cultured cartilage/bone cells/tissue, method of generating same and uses thereof
AssigneeTechnion Research & Development Foundation Limited
InventorGila Maor
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Abstract
A method of generating cultured chondrocytes/endochondral bone cells is provided. The method comprising isolating chondrocytes from mandibular condyle tissue, and culturing the isolated chondrocytes. A method of isolating chondrocytes from mandibular condyle tissue is further provided. The method comprises isolating mandibular condyle tissue from a mammal and treating the mandibular condyle tissue so as to selectively remove fibroblast-like cells and/or myocytes therefrom, the modified mandibular condyle tissue including chondrocytes, and selectively collecting the chondrocytes from the modified mandibular condyle tissue.
Claims19
A method of generating cultured chondrocytes, the method comprising:
- (a) isolating chondrocytes from mandibular condyle tissue of a neonatal mammal;
- and
- (b) culturing said isolated chondrocytes, wherein said culturing comprises plating said isolated chondrocytes as a monolayer and culturing said isolated chondrocytes for at least 7 days, thereby generating the cultured chondrocytes, wherein the cultured chondrocytes express collagen Type II and not collagen Type I.
A cell culture comprising a monolayer of chondrocytes isolated from a mandibular condyle tissue of a neonatal mammal
Dependencies are inferred from the claim text.