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US 2010/0135860 A1Published application
Holder for hole-type cell carrier
AssigneeCell Kinetics Ltd.
InventorsAsaf Halamis, Michael Sister
Life of this patent
Estimating the term…
Abstract
There are provided holders (10) for hole-type cell carriers (12) having structures that enable repeated contacting of cells with fluid from both above and below the cells. Other embodiments are also disclosed. The holders comprise a platform (52) for the cell carrier and a cover slip (46). Means to enable capillary flow of liquid between said platform and said cover slip are also provided.
Claims33
A holder for a hole-type cell carrier, the holder comprising:
- a body;
- an elongate platform on said body, said platform having sides and a first and a second end;
- a first hole located within said platform, into which a hole-type cell carrier can be emplaced so that a first surface of said hole-type cell carrier is substantially level with said platform;
- first and second flow-stopping structures arranged longitudinally on either side of said platform, each of said first and second flow stopping structures arranged at a height different than the level of said platform, each of said first and second flow stopping structures beginning closer to said first end of said platform than said first hole and terminating closer to said second end of said platform than said first hole;
- a first chamber formed in said body, said first chamber being located in fluid communication with said first hole at an end of said hole opposite to said platform;
- at least one well located adjacent to said platform, said well being of greater depth than said first hole and sized to contain a greater volume of fluid than said first hole;
- at least one of said flow-stopping structures terminating at a location so as to provide at least one opening where a fluid flowing over said platform can flow into said well;
- and
- at least one support structure capable of supporting a cover slip over said platform and spacing said cover slip from said platform at a distance which, when a liquid is place between said platform and said cover slip, will enable capillary flow of said liquid between said platform and said cover slip.
6 more elements
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6-9. (canceled)
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14-15. (canceled)
17-20. (canceled)
A holder suitable for use with a hole-type cell carrier, the holder comprising:
- a first face, the first face having thereupon a platform, said platform having sides and a first and a second end, a first hole in which a cell carrier can be mounted being formed within said platform, troughs arranged on either side of said platform, each of said troughs being connected to a well of greater depth than the trough to which the well is connected, a depression being formed in said platform between said first hole and said first end of said platform, and between said first hole and said second end of said platform a pair of spaced apart branches extending laterally from said platform, one of said pair of branches extending in the direction of one of said wells and the other of said pair of branches extending in the direction of the other of said wells, the spacing between each pair of branches increasing along the length of the branches, and, between each respective pair of branches, a declination from said platform into each respective well.
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29-31. (canceled)
A holder suitable for use with a hole-type cell carrier, the holder comprising:
- an upper face, the upper face having thereupon a first platform, said platform having sides and a first and second end;
- a first hole in which a cell carrier can be mounted being formed within said first platform;
- first and second ridges, having proximal and distal ends, arranged longitudinally on either side respectively of said first platform;
- a third ridge arranged transversely near a first end of said first platform;
- said first, second and third ridges rising to a uniform height above the level of said first platform;
- a depression formed near a second end of said first platform, such that said first hole is located between said depression and said third ridge;
- said distal end of said first ridge being located between the longitudinal position of said hole and said third ridge;
- a first well which is formed in the holder in the region adjacent to the side of said first platform upon which said first ridge is located, and which extends to a depth below the level of said first platform;
- said first platform being shaped so that, in the region of said first platform which is located between said third ridge and said distal end of said first ridge, said first platform has at least two maximal widths and at least one minimum width which is less than either of said maximal widths, said first platform reaching said maximal widths at (a) the place where said first platform is adjacent to said third ridge, and (b) the place where said first platform is adjacent to the distal end of said first ridge, said first platform projecting at said maximal widths in the direction of said first well.
6 more elements
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42-53. (canceled)
Dependencies are inferred from the claim text.