Decreasing RUBISCO content of algae and cyanobacteria cultivated in high carbon dioxide
AssigneeTransAlgae Ltd
InventorsDaniella Schatz, Jonathan Gressel, Doron Eisenstadt, Shai Ufaz
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Abstract
Algae and cyanobacteria are genetically engineered to have lower RUBISCO (ribulose-1,5-bisphosphate carboxylase/oxygenase) content in order to grow more efficiently at elevated carbon dioxide levels while recycling industrial CO2 emissions back to products, and so as not to be able to grow outside of cultivation.
Claims27
A method to over produce homologous or heterologous compounds in cyanobacteria or algae cells, said method comprising the steps of:
- a) Reducing amount of RUBISCO protein in the cells by genetically transforming the cells;
- b) Transforming the cells with a gene of interest under control of constitutive or inducible promoter to express production of the compound;
- and
- c) Cultivating the transgenic cell line in elevated carbon dioxide concentrations.
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A method to cultivate algae and cyanobacteria under high CO2 concentration, wherein the algae and cyanobacteria are genetically modified to express low amounts of RUBISCO protein.
A method to prevent establishment of transgenic algal or cyanobacterial cells in the natural environment by down regulating the amount of RUBISCO protein in the cells by genetic modification, whereby the cells become incapable to grow in ambient carbon dioxide concentrations.
A transgenic alga or cyanobacterium, transformed with a vector comprising rbcS or rbcL encoding polynucleotides in an antisense or an RNAi-construct under a constitutive promoter;
- whereby the transgenic alga or cyanobacterium expresses reduced content of RUBISCO protein.
Dependencies are inferred from the claim text.