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US 2010/0077514 A1Published application
Gene Expression Modulating Element
AssigneeE.I. du Pont de Nemours and Company
InventorsHochul Yi, Theodore M. Klein, Joanne E. Barton
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AssigneeE.I. du Pont de Nemours and Company
InventorsHochul Yi, Theodore M. Klein, Joanne E. Barton
Estimating the term…
The present invention provides a method of screening for the purpose of obtaining gene expression modulating elements and gene insulator elements. The invention includes a method of identifying gene expression modulating elements and gene insulator elements through use of the following steps: a) locating intergenic regions of a plant genome that are flanked by a gene on each side that have differing gene expressions b) taking that intergenic region or a portion of that intergenic region and adding it to a cassette comprising an isolated gene c) introducing the cassette into a plant cell d) analyzing expression of the isolated gene. The present invention also includes identified sequences that act as gene expression modulating elements.
An isolated nucleic acid comprising a gene expression modulating element of 40 to 200 base pairs operably linked to a heterologous polynucleotide of interest, wherein said gene expression modulating element is SEQ. ID NO. 1, and said gene modulating element influences the expression of the polynucleotide of interest.
Dependencies are inferred from the claim text.