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US 2010/0075860 A1Published application
Microassay with internal referencing
AssigneeWasatch Microfluidics, LLC
InventorsDavid Myszka, Bruce Kent Gale, Joshua Wayne Eckman, Sriram Natarajan
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Abstract
Specialized microfluidic networks are utilized to deposit substances on sensor surfaces. In particular, a flow-based microfluidic printhead is used as an interface to deliver multiple analytes to a sensor for simultaneous analysis. Furthermore, internal referencing is incorporated into sensor regions for improved sensitivity.
Claims34
A method for patterning a surface for a microassay, comprising:
- a) providing a spotter including at least one fluid pathway adapted to provide a printing cavity having a printing orifice and an interrogation cavity having an interrogation orifice, both the printing orifice and the interrogation orifice being configured to form a seal with the surface;
- b) placing the spotter against the surface so as to seal the printing orifice to the surface and form a printing flow chamber defined by the printing cavity and the surface;
- c) flowing a fluid through the printing flow chamber so as to print a spot onto the surface;
- d) relocating the spotter along the surface over a distance and in a direction so that the interrogation orifice overlaps both a portion of the spot and an adjacent unprinted space of the surface;
- and
- e) placing the spotter against the surface so as to seal the interrogation orifice to the surface to form an interrogation flow chamber defined by the interrogation cavity and a second location on the surface, said second location being positioned over both a portion of the spot and an adjacent unprinted space.
3 more elements
A method for performing a microassay with internal referencing, comprising:
- a) providing a spotter comprising a fluid pathway including a cavity that comprises an orifice adapted to form a seal with the surface;
- b) placing the spotter against the surface so as to seal the orifice to the surface and form a first printing flow chamber defined by the cavity and the surface;
- c) flowing a first fluid containing a first substance through the first printing flow chamber so as to print a first spot of the first substance onto the surface;
- d) relocating the spotter along the surface over an adjacent space relative to the first spot, and optionally partially over the first spot;
- e) placing the spotter against the surface so as to seal the orifice to the surface and form a second printing flow chamber defined by the cavity and the surface;
- f) flowing a second fluid containing a second substance through the second printing flow chamber so as to print the second substance to form a second spot;
- g) relocating the spotter along the surface so that the orifice overlaps both a portion of the first spot and a portion of the second spot;
- h) placing the spotter against the surface so as to seal the orifice to the surface and form an interrogation flow chamber defined by the cavity and the surface;
- and
- i) flowing a sample fluid through the interrogation flow chamber so that the sample fluid encounters both at least the portion of the first spot and the portion of the second spot.
7 more elements
A system for performing a microassay with internal referencing, comprising
- a) a spotter comprising a fluid pathway including a cavity with an orifice adapted to form a seal with a surface and to form a spot on the surface within the orifice;
- and
- b) a manipulator operably connected to the spotter and adapted to seal, relocate, and re-seal the orifice against the surface, said re-seal of the orifice on the substrate being carried out by relocating the cavity from a location of the seal over a distance and in a direction so that the orifice overlaps both a portion of the spot and an adjacent unprinted space of the surface.
A method of performing a microassay on surface, comprising:
- a) providing a spotter including at least one fluid pathway adapted to provide a printing cavity having a printing orifice and an interrogation cavity having an interrogation orifice, both the printing orifice and the interrogation orifice being configured to form a seal with the surface;
- b) placing the spotter against the surface so as to seal the printing orifice to the surface and form a printing flow chamber defined by the printing cavity and the surface;
- c) flowing a fluid including a capture substance through the printing flow chamber so as to print a spot including the capture substance onto the surface;
- d) relocating the spotter along the surface over a distance and in a direction so that the interrogation orifice overlaps both a portion of the spot and an adjacent unprinted space of the surface;
- e) placing the spotter against the surface so as to seal the interrogation orifice to the surface to form an interrogation flow chamber defined by the interrogation cavity and a second location on the surface, said second location being positioned over both a portion of the spot and an adjacent unprinted space;
- and
- f) flowing a sample fluid through the interrogation flow chamber so as to generate contact between the second fluid and both the portion of the spot and the unprinted space.
4 more elements
Dependencies are inferred from the claim text.