US 2010/0075395 A1 · MICROBIAL PROCESS FOR PRODUCTION OF ENZYMES

Abstract

The present invention relates to a suspension culture process for producing an enzyme, comprising the steps of providing a minimal medium, which contains deionised water, mineral salts and an organic carbon source, in a vessel; inoculating the minimal medium with a fungus; incubating the minimal medium at a pH value of 1-4 for a period of time which is sufficient for the fungus to be optically visible in the minimal medium, and obtaining the enzyme, wherein the minimal medium and the vessel are not sterilised. In addition, the invention relates to an enzyme obtainable by the process according to the invention.

Claims16

Dependent claims

A submerged culture process for producing an enzyme, comprising the steps of

a) providing a minimal medium having deionised water, mineral salts and an organic carbon source, in a vessel,
b) inoculating the minimal medium with a fungus,
c) incubating the minimal medium at a pH value of 1-4 for a period of time which is sufficient for the fungus to be optically visible in the minimal medium, and
d) obtaining the enzyme,
wherein the minimal medium and the vessel are not sterilised, and the fungus is Phialemonium spec. and/or Botrytis cinerea.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

12/517,766Filed 29 Oct 2007

Priority

DE 10 2006 057 724.84 Dec 2006

Published

25 Mar 2010

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12N 1/14
Microorganisms; Compositions thereof ; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of…:…
C12N 1/145
Fungi ; Culture media therefor: fungi isolates
C12N 9/00
Enzymes
C12N 9/20
Carboxylic ester hydrolases: triglyceride splitting, e.g. by means of lipase

Microbial process for production of enzymes

Life of this patent

Microbial process for production of enzymes

Life of this patent

Abstract

Claims16