Recombinant Yeast Strains Expressing Tethered Cellulase Enzymes
AssigneeTrustees of Dartmouth College
InventorsJohn E.E. McBride, Kristen M. Delault, Lee R. Lynd, Jack T. Pronk
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Abstract
Recombinant yeast strains that saccharify, ferment and grow on insoluble and crystalline forms of cellulose are disclosed herein. The yeast strains express tethered cellulases including cellobiohydrolase, endoglucanase and β-glucosidase. The recombinant organisms are particularly suited for consolidated bioprocessing.
Claims37
A transformed yeast cell that expresses a plurality of genes, wherein the genes code for expression of tethered enzymes including endoglucanase, cellobiohydrolase and β-glucosidase.
A transformed organism, comprising,
- a yeast that in a native state lacks the ability to saccharify cellulose, wherein the yeast is transformed with heterologous polynucleotides that express a plurality of enzymes that confer upon the yeast the ability to saccharify crystalline cellulose.
An isolated polynucleotide comprising:
- (a) a polynucleotide sequence of SEQ ID NO:
- 11;
- (b) a polynucleotide sequence of SEQ ID NO:
- 12;
- (c) a polynucleotide sequence of SEQ ID NO:
- 28;
- (d) a polynucleotide sequence of SEQ ID NO:
- 29;
- and
- (e) a polynucleotide sequence of SEQ ID NO:
- 30;
- or
- (f) a polynucleotide sequence having at least about 90% sequence identity with the polynucleotide sequences of (a)-(e).
A genetic construct comprising SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 28, SEQ ID NO:
- 29 and SEQ ID NO:
- 30 operably connected to promoters expressible in yeast.
Dependencies are inferred from the claim text.