Method for rapid identification and quantification of microorganisms
AssigneeThe Regents of the University of California
InventorsKathryn L. Creek, Alonso Castro, Perry C. Gray
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Abstract
Method for unamplified, selective identification of a microorganism comprising obtaining a sample comprising the microorganism, wherein the sample comprises unseparated genetic material of the microorganism; adding to the sample a molecular beacon comprising an nucleic sequence which is complementary to at least one nucleic acid sequence in the microorganism; and heating the sample; wherein the molecular beacon hybridizes with said nucleic acid sequence to produce a detectable signal.
Claims21
A method for unamplified, selective identification of a microorganism comprising:
- a) obtaining a sample comprising the microorganism, wherein the sample comprises unseparated genetic material of the microorganism;
- b) adding to the sample a molecular beacon comprising an nucleic sequence which is complementary to at least one nucleic acid sequence in the microorganism;
- and
- c) heating the sample;
- wherein the molecular beacon hybridizes with said nucleic acid sequence to produce a detectable signal.
A method for unamplified, selective identification of methicillin-resistant Staphylococcus aureus comprising:
- a) obtaining a sample comprising methicillin-resistant Staphylococcus aureus, wherein the sample comprises unseparated genetic material of the methicillin-resistant Staphylococcus aureus;
- b) adding to the sample a molecular beacon comprising a nucleic acid sequence selected from the group consisting of GCG GCT GCT GGC ACG TAG TTA (SEQ ID NO:
- 1), CCC GCG CGT CGC GAT TTC AAT ATG TAT GCT TTG GTC TTT CTG ATC GCG (SEQ ID NO:
- 3), and combinations thereof;
- and
- c) heating the sample;
- wherein the molecular beacon hybridizes a nucleic acid sequence in the organism of interest to produce a detectable signal.
Dependencies are inferred from the claim text.