Method and device
OwnerGesa RASCHER-EGGSTEIN, Thomas Knottinventors
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Abstract
A method of providing suspended cells (10) for carrying out biological assays, comprising the steps of: a) providing a frozen cell suspension, b) bringing the frozen cell suspension into direct contact with an excess volume of thawing buffer (12), and c) incubating the solution of step b) at room temperature in order to thaw the cell suspension.
Claims29
1-28. (canceled)
A method for providing suspended cells for carrying out biological assays, comprising the steps of:
- a) providing a frozen cell suspension,
- b) bringing the frozen cell suspension into direct contact with an excess volume of thawing buffer, and
- c) incubating the solution of step b) at room temperature in order to thaw the cell suspension.
A method for providing suspended cells for carrying out biological assays, comprising the steps of:
- a) providing a frozen cell suspension,
- b) bringing the frozen cell suspension into direct contact with an excess volume of thawing buffer, whereby the thawing buffer is present in the range of about 1:10 to about 1:40, and a frozen cell pellet is immersed directly in the thawing buffer which is present in a thawing vessel at approximately room temperature, and
- c) incubating the solution of step b) at room temperature in order to thaw the cell suspension.
A device for subjecting, in a storage vessel, cells in single-cell suspension to intermediate storage, which device comprises means for applying single-cell suspension maintaining measures to the solution in the storage vessel.
Dependencies are inferred from the claim text.