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AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsWilliam Allan Nix, M. Steven Oberste
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The present disclosure provides methods that permit detection of all known species of Parechovirus, including Human parechovirus and Ljungan virus (LV). In particular examples the method includes amplifying at least a portion of the 5′NTR of parechovirus nucleic acid molecules obtained from a sample, and detecting the resulting amplicons, but does not require culturing of the virus. The present disclosure also provides methods for determining which particular species or serotype of parechovirus is present in a biological sample. Also provided are oligonucleotide primers and probes that can be used in the disclosed methods.
A method of detecting Parechovirus species, comprising, contacting cDNA reverse transcribed from RNA isolated from a sample with a composition that permits amplification of the cDNA, wherein the composition comprises a forward primer and a first reverse primer, wherein the forward and first reverse primers hybridize to opposite strands of a parechovirus 5′ nontranslated region (5′NTR);
A method of identifying which Parechovirus species is present in the sample, comprising:
An isolated nucleic acid molecule consisting of any of SEQ ID NOS:
An isolated nucleic acid molecule comprising at least 95% sequence identity to SEQ ID NO:
An isolated nucleic acid molecule comprising at least 95% sequence identity to any of SEQ ID NOS:
Dependencies are inferred from the claim text.