G-csf polypeptides and uses thereof
AssigneeMerck Serono SA
InventorsCATHERINE GONTHIER, Philippe Millasseau
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Abstract
The present invention relates to G-CSF polypeptides and their uses, particularly for therapeutic or prophylactic treatment in human subjects. The invention also relates to nucleic acids encoding said polypeptides, vectors comprising such nucleic acids and recombinant cells containing the same. The invention further discloses methods of producing such polypeptides, as well as methods and tools for detecting or dosing these polypeptides in any sample.
Claims37
An isolated G-CSF polypeptide comprising:
- a) SEQ ID NO:
- 8;
- or
- b) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor.
An isolated nucleic acid molecule encoding a polypeptide comprising:
- a) SEQ ID NO:
- 8;
- b) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor;
- or
- c) a fusion protein comprising an additional amino acid domain operably linked to:
- i) SEQ ID NO:
- 8;
- or
- ii) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor.
A composition comprising a pharmaceutically acceptable carrier or diluent and a polypeptide comprising:
- a) SEQ ID NO:
- 8;
- b) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor;
- or
- c) a fusion protein comprising an additional amino acid domain operably linked to:
- i) SEQ ID NO:
- 8;
- or
- ii) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor.
A method of producing a polypeptide comprising culturing a recombinant host cell under conditions allowing expression of the nucleic acid molecule, and recovering the polypeptide produced, wherein said recombinant host cell comprises a nucleic acid encoding:
- a) SEQ ID NO:
- 8;
- b) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor;
- or
- c) a fusion protein comprising an additional amino acid domain operably linked to:
- i) SEQ ID NO:
- 8;
- or
- ii) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor.
A method of stimulating the immune system in a subject comprising the administration of a composition comprising:
- a) SEQ ID NO:
- 8;
- b) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor;
- or
- c) a fusion protein comprising an additional amino acid domain operably linked to:
- i) SEQ ID NO:
- 8;
- or
- ii) a variant of SEQ ID NO:
- 8 comprising one or several amino acid substitutions to SEQ ID NO:
- 8, wherein said variant binds a G-CSF receptor.
Dependencies are inferred from the claim text.