Nucleic acid separation and purification method based on reversible charge interactions
OwnerNurith Kurn, Joe Don Heathinventors
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Abstract
The invention provides a method for purifying nucleic acids using a polycationic reagent and an anionic substrate to form a complex with a nucleic acid to be purified. The complex may be separated from other components of a mixture and the nucleic acid eluted from the complex with a high ionic strength solution or an anionic reagent.
Claims34
A method for purifying a nucleic acid, comprising:
- contacting a sample or reaction mixture comprising said nucleic acid with a polycationic reagent and an anionic substrate, wherein a nucleic acid-polycation-anionic substrate complex is formed;
- separating said nucleic acid-polycation-anionic substrate complex from other components of said sample or reaction mixture;
- and
- eluting said nucleic acid from said nucleic acid-polycation-anionic substrate complex.
A method according to claim 27, wherein said fragment is about 10 to about 200 nucleotides in length.
A method for purifying a nucleic acid, comprising:
- separating a nucleic acid-polycation, anionic substrate complex from other components of a sample or reaction mixture, wherein said complex is produced by contacting a sample or reaction mixture comprising said nucleic acid with a polycationic reagent and an poly-anionic substrate;
- and
- eluting said nucleic acid from said nucleic acid-polycation-anionic substrate complex.
A kit for nucleic acid purification, comprising a polycationic reagent, an anionic substrate, and a buffer suitable for formation of a nucleic acid-polycation-anionic substrate complex.
Dependencies are inferred from the claim text.