Long-acting epo polypeptides and derivatives thereof and methods thereof
AssigneeProlor Biotech Ltd.
InventorsFUAD FARES, UDI EYAL FIMA
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Abstract
A polypeptide and polynucleotides encoding same comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to an EPO peptide are disclosed. Pharmaceutical compositions comprising the polypeptide and polynucleotides of the invention and methods of using same are also disclosed.
Claims22
A polynucleotide molecule comprising a DNA sequence, wherein said a DNA sequence consists a coding portion encoding a polypeptide, said polypeptide consists of an EPO polypeptide, one chorionic gonadotrophin carboxy terminal peptide attached to the amino terminus of said EPO polypeptide, and two chorionic gonadotrophin carboxy terminal peptides attached to the carboxy terminus of said EPO polypeptide.
A polynucleotide molecule comprising a DNA sequence, wherein said a DNA sequence consists a coding portion encoding a polypeptide, said polypeptide consists of an EPO polypeptide, one chorionic gonadotrophin carboxy terminal peptide attached to the amino terminus of said EPO polypeptide, two chorionic gonadotrophin carboxy terminal peptides attached to the carboxy terminus of said EPO polypeptide, and a signal peptide attached to the amino terminus of said one chorionic gonadotrophin carboxy terminal peptide.
A method of producing an EPO polypeptide with an improved biological activity in a cell, comprising the step of transfecting said cell with an expression vector comprising a coding portion encoding a polypeptide, said polypeptide comprises an EPO polypeptide and at least two chorionic gonadotrophin carboxy terminal peptides, wherein a first chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to an amino terminus of said EPO polypeptide, and a second chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to the carboxy terminus of said EPO polypeptide, thereby producing an EPO polypeptide with an improved biological activity in a cell.
A method of producing an EPO polypeptide with an improved biological half life and an improved biological activity in a cell, comprising the step of transfecting said cell with an expression vector comprising a coding portion encoding a polypeptide, said polypeptide comprises an EPO polypeptide and at least two chorionic gonadotrophin carboxy terminal peptides, wherein a first chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to an amino terminus of said EPO polypeptide, and a second chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to the carboxy terminus of said EPO polypeptide, thereby producing an EPO polypeptide with an improved biological half life and an improved biological activity in a cell.
Dependencies are inferred from the claim text.