Novel Nucleoside or Nucleotide Derivative and Use Thereof
AssigneeRiken
InventorsIchiro Hirao, Shigeyuki Yokoyama
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Abstract
The object of the present invention is to provide a nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, as well as a method using the same. In one embodiment, the nucleoside or nucleotide of the present invention has a fluorescent dye selected from the group consisting of 5-FAM, 6-FAM, 5-TAMRA, 6-TAMRA, DANSYL, 5-HEX, 6-HEX, 5-TET, 6-TET, 5-ROX and 6-ROX or a quencher dye selected from the group consisting of DABCYL, BHQ1 and BHQ2, which is attached either directly or through a linker to the 5-position of the above base.
Claims15
A nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, which has a fluorescent dye attached either directly or through a linker to the 5-position of the base, wherein the fluorescent dye is selected from the group consisting of 5-carboxyfluorescein (5-FAM), 6-carboxyfluorescein (6-FAM), 5-carboxytetramethylrhodamine (5-TAMRA), 6-carboxytetramethylrhodamine (6-TAMRA), 5-(dimethylamino)naphthalene-1-sulfonic acid (DANSYL), 5-carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein (5-HEX), 6-carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein (6-HEX), 5-carboxy-2′,4,7,7′-tetrachlorofluorescein (5-TET), 6-carboxy-2′,4,7,7′-tetrachlorofluorescein (6-TET), 5-carboxy-X-rhodamine (5-ROX), and 6-carboxy-X-rhodamine (6-ROX).
A nucleoside or nucleotide having a 5-substituted-2-oxo(1H)pyridin-3-yl group as a base, which has a quencher dye attached either directly or through a linker to the 5-position of the base, wherein the quencher dye is selected from the group consisting of 4-(4-dimethylaminophenylazo)benzoic acid (DABCYL), N-methyl-N-[4-[2-methoxy-5-methyl-4-(2-nitro-4-methylphenylazo)phenylazo]phenyl]-4-aminobutyric acid (BHQ1) and N-methyl-N-[4-[2,5-dimethoxy-4-(4-nitrophenylazo)phenylazo]phenyl]-4-aminobutyric acid (BHQ2).
Dependencies are inferred from the claim text.