Long-acting polypeptides and methods of producing same
AssigneeProlor Biotech Ltd.
InventorsFUAD FARES, UDI EYAL FIMA
Life of this patent
Estimating the term…
Abstract
A polypeptide and polynucleotides encoding same comprising at least two carboxy-terminal peptides (CTP) of chorionic gonadotrophin attached to a peptide-of-interest are disclosed. Pharmaceutical compositions comprising the polypeptide and polynucleotides of the invention and methods of using same are also disclosed.
Claims22
A polynucleotide molecule comprising a DNA sequence, wherein said DNA sequence consists a coding portion encoding a polypeptide, said polypeptide consists of a hGH polypeptide, one chorionic gonadotrophin carboxy terminal peptide attached to the amino terminus of said hGH polypeptide, and two chorionic gonadotrophin carboxy terminal peptides attached to the carboxy terminus of said hGH polypeptide.
A polynucleotide molecule comprising a DNA sequence, wherein said a DNA sequence consists a coding portion encoding a polypeptide, said polypeptide consists of a hGH polypeptide, one chorionic gonadotrophin carboxy terminal peptide attached to the amino terminus of said hGH polypeptide, two chorionic gonadotrophin carboxy terminal peptides attached to the carboxy terminus of said hGH polypeptide, and a signal peptide attached to the amino terminus of said one chorionic gonadotrophin carboxy terminal peptide.
A method of producing a hGH polypeptide with an improved biological activity in a cell, comprising the step of transfecting said cell with an expression vector comprising a coding portion encoding a polypeptide, said polypeptide comprises a hGH polypeptide and at least two chorionic gonadotrophin carboxy terminal peptides, wherein a first chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to an amino terminus of said hGH polypeptide, and a second chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to the carboxy terminus of said hGH polypeptide, thereby producing a hGH polypeptide with an improved biological activity in a cell.
A method of producing a hGH polypeptide with an improved biological half life and an improved biological activity in a cell, comprising the step of transfecting said cell with an expression vector comprising a coding portion encoding a polypeptide, said polypeptide comprises a hGH polypeptide and at least two chorionic gonadotrophin carboxy terminal peptides, wherein a first chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to an amino terminus of said hGH polypeptide, and a second chorionic gonadotrophin carboxy terminal peptide of said at least two chorionic gonadotrophin carboxy terminal peptides is attached to the carboxy terminus of said hGH polypeptide, thereby producing a hGH polypeptide with an improved biological half life and an improved biological activity in a cell.
Dependencies are inferred from the claim text.