Mashing Process
AssigneeNovozymes A/S
InventorsRikke Monika Festersen, Anders Viksoe Nielsen, Christel Thea Joergensen, Lars Lehmann Hylling Christensen
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Abstract
The present invention relates to a mashing and filtration step in a brewing process and to a composition useful in the mashing and filtration step of a brewing process.
Claims21
1-27. (canceled)
A process for production of a mash having enhanced filterability and/or improved extract yield after filtration, which comprises;
- preparing a mash in the presence of enzyme activities and filtering the mash to obtain a wort, wherein the enzyme activities comprise;
- a xylanase of GH family 10 present in an amount of at least 15% w/w of the total xylanase and endoglucanase enzyme protein of said composition.
The process of claim 1 wherein endoglucanase is present, said endoglucanase belonging to a GH family selected from the list consisting of;
- GH12, GH7 and GH5.
The process of claim 1 wherein the endoglucanase activity belonging to GH family GH12, GH7 and/or GH5 is present in an amount of at least 40% w/w of the total xylanase and endoglucanase enzyme protein of said composition.
The process of claim 1 wherein the xylanase of GH family 10 is present in an amount of at least 20%, preferably 25%, such as at least 30%, at least 35%, at least 40%, at least 45%, at least 50%, at least 60%, or even at least 70% w/w of the total xylanase and endoglucanase enzyme protein
The process of claim 1 wherein the endoglucanase of GH Family 12, 7 and/or 5 endoglucanase is present in an amount of at least 45%, preferably 50%, such as at least 55%, at least 60%, at least 70% or even at least 80% w/w of the total xylanase and endoglucanase enzyme protein.
The process of claim 1 wherein the xylanase is a type A xylanase.
The process of claim 1 wherein the xylanase is a type A xylanase having a |1,3terminal/|1,3internal ratio of at least 0.25, such as at least 0.30, al least 0.40, at least 0.50, or even at least 0.60.
The process of claim 1 wherein the xylanase has a CBM, preferably a CBM of family 1.
The process of claim 1 wherein the xylanase is a xylanase which in the xylanase binding assay described herein has a barley soluble/insoluble fibre binding ratio of at least 0.50, preferably at least 0.60, more preferably at least 0.70, such as 0.80, 0.90, 1.00, 1.10 or even at least 1.20.
The process of claim 1 wherein the xylanase is a xylanase derived from a filamentous fungi such as from a strain of an Aspergillus sp., preferably from Aspergillus aculeatus (SEQ ID NO:8 or SEQ ID NO:9), from a strain of a Myceliophotora sp., preferably from a Myceliophotora thermophilia (SEQ ID NO:13), from a strain of a Humicola sp., preferably from Humicola insolens (SEQ ID NO:12), or from a strain of Trichoderma sp., preferably from T reesei (SEQ ID NO:17).
The process of claim 1 wherein the xylanase is derived from a bacterium such as from a strain of a Bacillus.
The process of claim 1 wherein the endoglucanase is;
- an endoglucanase derived from Humicola sp., such as the endoglucanase from Humicola insolens (SEQ ID NO:3), or the endoglucanase from H. insolens (SEQ ID NO:4), from Thermoascus sp., such as the endoglucanase derived from Thermoascus aurantiacus (SEQ ID NO:6) or from Aspergillus sp., such as the endoglucanase derived from Aspergillus aculeatus (SEQ ID NO:16) or from Trichoderma sp., such as the endoglucanase from T. reseei shown in SEQ ID NO:18, the endoglucanase from T. viride sp. shown in SEQ ID NO:19 or the endoglucanase from T. reseei shown in SEQ ID NO:20.
The process of claim 1 wherein at least one additional enzyme is present, which enzyme is selected from the list comprising;
- arabinofuranosidase, ferulic acid esterase and xylan acetyl esterase.
A process of reducing the viscosity of an aqueous solution comprising a starch hydrolysate, said process comprising:
- a. testing at least one xylanolytic enzyme for its hydrolytic activity towards insoluble wheat arabinoxylan,
- b. selecting a xylanolytic enzyme which cleaves next to branched residues thereby leaving terminal substituted xylose oligosaccharides.
- c. adding the selected xylanolytic enzyme to the aqueous solution comprising a starch hydrolysate.
A process of reducing the viscosity of an aqueous solution comprising a starch hydrolysate, said process comprising:
- d. testing at least one endoglucanolytic enzyme for its hydrolytic activity towards barley beta-glucan,
- e. selecting a endoglucanolytic enzyme which under the conditions:
- 10 microgram/ml purified enzyme and 5 mg/ml barley beta-glucan in 50 mM sodium acetate, 0.01% Triton X-100, at pH 5.5 and 50° C., within 1 hour degrades more than 70% of the barley beta-glucan to DP 6 or DP<6,
- f. adding the selected endoglucanolytic enzyme to the aqueous solution comprising a starch hydrolysate.
The process claim 15, wherein the aqueous solution comprising a starch hydrolysate is a mash for beer making or a feed composition
A composition comprising;
- g. a GH10 xylanase present in an amount of at least 15% w/w of the total enzyme protein;
- and/or,
- h. a GH12, GH7 and/or GH5 endoglucanase present in an amount of at least 20% w/w of the total enzyme protein.
The composition according to the preceding claims wherein the xylanase is derived from a bacterium such as from a strain of a Bacillus.
Dependencies are inferred from the claim text.