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US 2009/0269809 A1Published application
Thermostable ribonuclease h
AssigneeTakara Bio Inc.
InventorsShigekazu Hokazono, Takashi Uemori, Tetsuki Tanaka, Ikunoshin Kato
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Abstract
A polypeptide having an RNaseH activity and being highly useful in gene engineering; a gene encoding this polypeptide; and a genetic engineering process for producing the polypeptide.
Claims4
A nucleic acid encoding a polypeptide having a thermostable ribonuclease H activity, said nucleic acid being selected from the group consisting of:
- (a) a nucleic acid encoding a polypeptide having the amino acid sequence of SEQ ID NO:1;
- (b) a nucleic acid encoding a polypeptide having an amino acid sequence in which at least one amino acid residue is deleted, added, inserted or substituted in the amino acid sequence of SEQ ID NO:1;
- and
- (c) a nucleic acid encoding a polypeptide having an amino acid sequence that shares at least 54% homology with the amino acid sequence of SEQ ID NO:1;
- (d) a nucleic acid having the nucleotide sequence of SEQ ID NO:2;
- (e) a nucleic acid consisting of a nucleotide sequences in which at least one nucleotide is deleted, added, inserted or substituted in the nucleotide sequence of SEQ ID NO:2 such that the deletion, addition, insertion or substitution of the nucleotide results in translation into an amino acid sequences;
- (f) a nucleic acid capable of hybridizing to any one of the nucleic acids of (a) to (d) or complementary strands thereof under stringent conditions;
- and
- (g) a nucleic acid having a nucleotide sequence that shares at least 61% homology with the nucleotide sequence of SEQ ID NO:2.
6 more elements
Dependencies are inferred from the claim text.