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OwnerAnne M. Gardner, Paul R. Gardnerinventors
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A protein expression vector that expresses large quantities of recombinant proteins under anoxic or microaerobic conditions by inducing expression with nitrate. The vector backbone is pUC19 and protein expression is driven by the E. coli flavohemoglobin promoter, which is inducible by nitrate, nitrite, or nitric oxide under conditions of low oxygen. The Nde1 site of pUC19 has been destroyed by filling in with Klenow fragment and religating the vector. An Nde1 site in the promoter provides an in-frame start methionine and a standard polylinker is available for ease of subcloning. The vector is named pANX for Plasmid ANaerobic eXpression.
A vector to express recombinant proteins, comprising the nucleic acid sequence SEQ. ID. NO. 2, wherein said proteins are expressed under microaerobic or anoxic conditions.
A vector to express recombinant proteins under microaerobic or anoxic conditions, said vector including a promoter that is inducible by nitrate, nitrite, or nitric oxide.
A method of expressing a recombinant protein under anoxic or microaerobic conditions, comprising:
A method of generating a nitrate inducible expression vector comprising:
Dependencies are inferred from the claim text.