Enzyme mediated delivery system
AssigneeAppian Labs, LLC
InventorsJ. Brian Windsor, Nitin Nitin
Life of this patent
Estimating the term…
Abstract
The present invention includes compositions, methods, and systems for the development of a novel delivery vehicle that affects release of an agent upon the degradation of components of said vehicle by one or more enzymes. In one example, the system comprises components designed to degrade upon the presence of desired concentrations of proteinases, specifically matrix metalloproteinases, and subsequent release of the agent.
Claims41
A method of controlling the release of an active agent with a proteinase comprising:
- encapsulating one or more active agents with a crosslinked or uncrosslinked matrix, the matrix being cleavable by the proteinase;
- wherein exposure of the matrix to one or more proteinases causes the cleavage of the matrix and the release of the active agents.
A tunable system for delivery of agents comprising a matrix comprising an active agent and a substrate for a proteinase enzyme and which, upon exposure to the proteinase is degraded thereby triggering the release of the active agent.
A method of making a composition that controls the release of an active agent upon exposure to a metalloproteinase comprising:
- encapsulating one or more active agents with a polypeptide-matrix encapsulant comprising cleavable polypeptides that are susceptible to cleavage by the metalloproteinase and a polymer;
- wherein exposure of the encapsulant to one or more metalloproteinases triggers the cleavage of the polypeptide and the release of the active agents.
A composition for the controlled release of an active agent comprising:
- a matrix comprising a polymeric portion crosslinked by a polypeptide that is susceptible to cleavage by a metalloproteinase, wherein erosion of the matrix by exposure to a metalloproteinase that is specific for the protein causes the release of one or more active agents encapsulated by the matrix.
A composition for the controlled release of an active agent made by a method comprising:
- encapsulating one or more active agents with a polymer-polypeptide matrix, wherein the polypeptide are susceptible to cleavage by metalloproteinases;
- and
- exposing the polymer-polypeptide matrix to one or more metalloproteinases, wherein the cleavage of the polypeptide causes the release of the encapsulated active agents.
Dependencies are inferred from the claim text.