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US 2009/0269306 A1Published application
N-terminal modified interferon-alpha
AssigneePharmaEssentia Corp.
InventorsBryan T.H. Wu, Tsai-Kuei Shen, Ming-Pin Hsu, Ching-Leou Teng
Life of this patent
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Abstract
A method of reducing formation of non-natural disulfide bonds in a mature IFN-α by adding one or more amino acid residues to its N-terminus cystein. Also disclosed herein is the IFN-α thus modified.
Claims20
An isolated polypeptide comprising
- a first fragment represented by Xn, X being an amino acid residue and n being 1, 2, 3, 4, or 5, in which if n is 1, X is not Met;
- and
- a second fragment including a mature interferon alpha, the N-terminus of which is Cys, the mature interferon alpha forming the N-terminal portion of the second fragment, wherein the Cys is linked to the first fragment via a peptide bond.
An isolated nucleic acid comprising a nucleotide sequence that encodes a polypeptide including:
- a first fragment represented by Xn, X being an amino acid residue and n being 1, 2, 3, 4, or 5, in which if n is 1, X is not Met;
- and
- a second fragment including a mature interferon alpha, the N-terminus of which is Cys, the mature interferon alpha forming the N-terminal portion of the second fragment, wherein the Cys is linked to the first fragment via a peptide bond.
A method of reducing formation of non-naturally occurring disulfide bonds in interferon alpha, comprising adding one to five amino acid residues to the N-terminus Cys of a mature interferon alpha.
Dependencies are inferred from the claim text.