Methods for treating, preventing and diagnosing procine ttv infection
OwnerJohn A. Ellisinventor
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Abstract
Compositions and methods for treating, preventing and diagnosing Torque teno virus (TTV) infection in pigs are disclosed. Also described are methods of identifying compounds for the treatment and prevention of TTV infection, as well as TTV animal models and methods of producing the same.
Claims25
A composition comprising a pharmaceutically acceptable vehicle and at least one porcine Torque teno virus (TTV) immunogen selected from the group consisting of an inactivated immunogenic porcine TTV, an attenuated immunogenic porcine TTV and an isolated immunogenic porcine TTV polypeptide.
A method of producing a composition comprising:
- (a) providing at least one porcine Torque teno virus (TTV) immunogen selected from the group consisting of an inactivated immunogenic porcine TTV, an attenuated immunogenic porcine TTV and an isolated immunogenic porcine TTV polypeptide;
- and
- (b) combining said TTV immunogen with a pharmaceutically acceptable vehicle.
A method of detecting Torque teno virus (TTV) infection in a vertebrate subject comprising:
- (a) providing a biological sample from the subject;
- and
- (b) reacting said biological sample with at least one isolated immunogenic porcine TTV polypeptide, under conditions which allow TTV antibodies, when present in the biological sample, to bind with said TTV polypeptide, thereby detecting the presence or absence of TTV infection in the subject.
A method for infecting a young TTV-negative piglet, a barrier-raised specific pathogen-free piglet, or caesarian-delivered piglet with a porcine isolate of Torque teno virus (TTV), said method comprising:
- (a) isolating TTV from a porcine subject;
- and
- (b) administering a dose of the TTV isolate to said piglet in an amount sufficient to cause TTV infection.
A method for evaluating the ability of a vaccine to prevent Torque teno virus (TTV) infection comprising:
- (a) administering to a porcine subject a candidate vaccine;
- (b) exposing the porcine subject from step (a) to a porcine TTV isolate in an amount sufficient to cause infection in an unvaccinated subject;
- and
- (c) observing the incidence of TTV infection in the porcine subject, thereby evaluating the ability of the candidate vaccine to prevent TTV infection.
A method for evaluating the ability of a vaccine to prevent a porcine circovirus-associated disease (PCVAD) comprising:
- (a) administering to a porcine subject a candidate vaccine;
- (b) exposing the porcine subject from step (a) to a porcine Torque teno virus (TTV) isolate in an amount sufficient to cause infection in an unvaccinated subject;
- and
- (c) observing the incidence of a PCVAD in the porcine subject, thereby evaluating the ability of the candidate vaccine to prevent PCVAD.
A method of identifying a compound capable of treating a porcine Torque teno virus (TTV) infection, said method comprising:
- (a) exposing a young TTV-negative piglet, a barrier-raised specific pathogen-free piglet, or caesarian-delivered piglet to a porcine TTV isolate in an amount sufficient to cause infection in said piglet;
- (b) delivering a compound or series of compounds to said infected piglet;
- and
- (c) examining the piglet from step (b) for the presence or loss of TTV and/or the development, inhibition, or amelioration of PCVAD symptoms relative to an untreated TTV-infected piglet.
Dependencies are inferred from the claim text.