Loading patent…
OwnerMichi Nakata, Noel Clark +2inventors
InventorsMichi Nakata, Noel Clark, Tommaso Bellini, Giuliano Zanchetta
Estimating the term…
Upon cooling solutions or melts of nucleic acids or other polymers in which the constituent molecules exhibit a varying tendency to form multi-strand complexes and thus liquid crystal phases, the complex-forming oligomers preferentially separate into the liquid crystal phase and condensing into liquid crystal domains. Because the tendency for liquid crystal phase formation correlates with the ability of the single strands to form duplexes, which in turn, depends on their degree of complementarity, separation of the liquid crystal domains thus constitutes a method for separating these molecules based upon their degree of complementarity.
A material comprising polynucleotide molecules, said material exhibiting at least one liquid crystal domain and at least one non-liquid crystal domain, wherein both the liquid crystal domain and the non-liquid crystal domain coexist and contain at least one polynucleotide molecule.
A material comprising polynucleotide molecules less than 100 base pairs in length, said material exhibiting at least one liquid crystal domain and at least one non-liquid crystal domain, wherein both the liquid crystal domain and the non-liquid crystal domain coexist and contain at least one polynucleotide molecule.
A solution or melt of polynucleotide molecules less than 100 base pairs in length, said solution or melt exhibiting at least one liquid crystal domain and at least one non-liquid crystal domain, wherein both the liquid crystal domain and the non-liquid crystal domain coexist and contain at least one polynucleotide molecule.
A polynucleotide complex comprising:
A method for separating polynucleotide molecules capable of forming a liquid crystal phase from a mixture of polynucleotide molecules wherein some molecules are capable of forming a liquid crystal phase and some molecules are not capable of forming a liquid crystal phase, comprising the steps of:
A method for identifying a target polynucleotide molecule within a population of polynucleotide molecules, comprising the steps of:
A method of separating a heterogeneous populations of molecules in a solution of melt, said molecules exhibiting varying degrees of tendency for forming multi-molecular complexes in a liquid crystal phase, said separation being effected by separation of molecules in the liquid crystal phase from molecules in the non-liquid crystal phase of said solution or melt.
An oligonucleotide having a sequence selected from the group consisting of SEQ ID Nos. 1-13.
Dependencies are inferred from the claim text.