Three-dimensional image obtaining device and processing apparatus using the same

A three-dimensional processing apparatus, comprising a three-dimensional image obtaining device, in which a biological material that is almost transparent is mounted as a specimen, for obtaining a three-dimensional image from an attenuation amount of a transmitted light through the specimen, specifying position coordinates of an arbitrary point in a specimen space of a biological specimen that is almost transparent, and optically stimulating and/or optically processing the arbitrary point,

• wherein the three-dimensional image obtaining device comprises:
• a light source emitting an optical flux with light intensity being amplitude modulated by a predetermined frequency;
• an objective lens focusing the optical flux at a measurement point in the specimen to generate a local intensity distribution in the vicinity of the measurement point;
• a means for three-dimensionally scanning the specimen and the measurement point relatively;
• a modulated low voltage signal processor calculating a difference between a reference signal voltage equivalent to a transmitted light amount of the optical flux of when there is no specimen and a specimen transmitted signal voltage equivalent to a transmitted light amount of the optical flux of when there is a specimen, and outputting a modulated signal voltage equivalent to a minute light attenuation amount of the optical flux corresponding to position coordinates of the measurement point to be scanned;
• a means for making a three-dimensional map using the modulated signal voltage corresponding to the position coordinates of the measurement point;
• and
• a means for making a three-dimensional image of the specimen from the three-dimensional map by either a deconvolution using a convolution kernel obtained from a light intensity distribution image in the vicinity of the measurement point or a blind deconvolution which does not initially set a convolution kernel.