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AssigneeCargill, Incorporated
InventorsMatthew Miller, Pirkko Suominen, Aristos Aristidou, Benjamin Matthew Hause, Pim Van Hoek, Catherine Asleson Dundon
Estimating the term…
Yeast cells having an exogenous lactate dehydrogenase gene ae modified by reducing L- or D-lactate:ferricytochrome c oxidoreductase activity in the cell. This leads to reduced consumption of lactate by the cell and can increase overall lactate yields in a fermentation process. Cells having the reduced I, or D-lactate:ferricytochrome c oxidoreductase activity can be screened for by resistance to organic acids such as lactic or glycolic acid.
A genetically modified yeast cell having at least one functional exogenous lactate dehydrogenase (LDH) gene which yeast cell lacks the ability to grow on D-lactate, L-lactate or both D-lactate and L-lactate, as a sole carbon source.
A genetically modified yeast cell having at least one functional exogenous lactate dehydrogenase gene and a reduced L- or D-lactate:ferricytochrome c oxidoreductase activity, compared to that of a wild-type stain.
A genetically modified yeast cell having (a) at least one functional exogenous lactate dehydrogenase (LDH) gene integrated into its genome and (b) a deletion or disruption of (i) at least one native L- or D-lactate:ferricytochrome c oxidoreductase gene.
19-24. (canceled)
27-33. (canceled)
A cell having (1) a functional L-lactate:ferricytochrome c oxidoreductase (CYB2) cassette integrated into its genome at a location other than the locus of a CYB2 gene that is native to the cell and (2) a deletion or disruption of at least one CYB2 gene that is native to the cell.
Dependencies are inferred from the claim text.