Cellular Pyrogen Test
AssigneeFraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V.
InventorsHerwig Brunner, Doris Finkelmeier, Georg Geiger, Anke Burger-Kentischer
Life of this patent
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Abstract
The invention concerns methods, agents and kits for qualitative and quantitative detection and identification of pathogens and pathogen spectra based on endotoxins and other pyrogens.
Claims63
Transgenic cell for specific detection of a pyrogen in a sample containing in the genome:
- a) a gene or genes that code for at least one toll-like receptor (TLR) and
- b) at least one reporter gene under the expression control of a promoter inducible by
- NF-κB.
Cell according to one of the preceding claims, in which the cell contains a gene or genes that code for a first toll-like receptor type (TLR type) and additionally a gene or genes that codes for second toll-like receptor type (TLR type).
Cell according to one of the preceding claims, in which the cells additionally have a gene that codes for the CD14 receptor.
Cell according to one of the preceding claims, in which the reporter gene codes for a secreted alkaline phosphatase (SEAP).
Cell according to one of the preceding claims, in which the reporter gene codes for a β-galactosidase.
Cell according to one of the preceding claims, in which the reporter gene codes for a luciferase.
Cell according to one of the preceding claims, in which the reporter gene codes for GFP.
Cell according to one of the preceding claims, in which the inducible promoter is the promoter for selectin (endothelial cell leukocyte adhesion molecule;
- ELAM-1).
Cell according to one of the preceding claims, which expresses the human TLR type 1 (TLR-1).
Cell according to one of the preceding claims, which expresses the human TLR type 2 (TLR-2).
Cell according to one of the preceding claims, which expresses the human TLR type 3 (TLR-3).
Cell according to one of the preceding claims, which expresses the human TLR type 4 (TLR-4).
Cell according to one of the preceding claims, which expresses the human TLR type 5 (TLR-5).
Cell according to one of the preceding claims, which expresses the human TLR type 6 (TLR-6).
Cell according to one of the preceding claims, which expresses the human TLR type 7 (TLR-7).
Cell according to one of the preceding claims, which expresses the human TLR type 8 (TLR-8).
Cell according to one of the preceding claims, which expresses the human TLR type 9 (TLR-9).
Cell according to one of the preceding claims, which expresses the human TLR type 10 (TLR-10).
Cell according to one of the preceding claims, which expresses the heterodimeric receptor for the human TLR type 1 (TLR-1) and human TLR type 2 (TLR-2).
Cell according to one of the preceding claims, which expresses the heterodimeric receptor for the human TLR type 6 (TLR-6) and human TLR type 2 (TLR-2).
Cell according to one of the preceding claims, which expresses the heterodimeric receptor for the human TLR type 7 (TLR-7) and human TLR type 8 (TLR-8).
Cell according to one of the preceding claims, which additionally expresses the coreceptor type CD14 (MD2).
Kit for specific detection of a pyrogen in the sample containing:
- a culture vessel with at least one transgenic cell according to one of the preceding claims.
Dependencies are inferred from the claim text.