Method for modifying plant morphology, biochemistry and physiology
OwnerThomas Schmulling, Tomas Wernerinventors
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OwnerThomas Schmulling, Tomas Wernerinventors
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The present invention relates to methods for stimulating root growth and/or enhancing the formation of lateral or adventitious roots and/or altering root geotropism comprising expression of a cytokinin oxidase or comprising expression of another protein that reduces the level of active cytokinins in plants or plant parts. Also provided by the present invention are methods for increasing seed size and/or weight, embryo size and/or weight, and cotyledon size and/or weight. The methods comprise expression of a cytokinin oxidase or expression of another protein that reduces the level of active cytokinins in plants or plant parts. Methods and compositions for increasing seed yield are also provided. The invention also relates to isolated plant cytokinin oxidase proteins, nucleic acid sequences encoding cytokinin oxidase proteins as well as to vectors, host cells, transgenic cells and plants comprising such sequences. The use of these sequences for improving root-related characteristics including increasing yield and/or enhancing early vigor and/or modifying root/shoot ratio and/or improving resistance to lodging and/or increasing drought tolerance and/or promoting in vitro propagation of explants and/or modifying cell fate and/or plant development and/or plant morphology and/or plant biochemistry and/or plant physiology, is also provided. The invention also relates to methods for identifying and obtaining proteins and compounds interacting with cytokinin oxidase proteins as well as the use of such proteins and/or compounds as plant growth regulators or herbicides.
A method for increasing seed yield in a plant, said method comprising expression of an isolated nucleic acid molecule encoding a cytokinin oxidase in a seedling or in the endosperm, embryo or aleurone of a plant seed.
An isolated nucleic acid molecule encoding a cytokinin oxidase operably linked to an endosperm-preferred promoter.
An isolated nucleic acid molecule encoding a cytokinin oxidase operably linked to a seedling-preferred promoter.
A vector comprising the isolated nucleic acid molecule having the sequence set forth in SEQ ID NO:42 or SEQ ID NO:47.
A vector comprising the isolated nucleic acid molecule having the sequence set forth in SEQ ID NO:43.
Dependencies are inferred from the claim text.