US 2009/0222947 A1 · Plants Modified With Mini-Chromosomes

Abstract

The invention is generally related to methods of generating plants transformed with novel autonomous mini-chromosomes. Mini-chromosomes with novel compositions and structures are used to transform plants cells which are in turn used to generate the plant. Methods for generating the plant include methods for delivering the mini-chromosome into plant cell to transform the cell, methods for selecting the transformed cell, and methods for isolating plants transformed with the mini-chromosome. Plants generated in the present invention contain novel genes introduced into their genome by integration into existing chromosomes.

Claims71

Dependent claims

An isolated mini-chromosome comprising a centromere, wherein the centromere comprises

(a) at least two first repeated nucleotide sequences that hybridize under conditions comprising 0.5×SSC and 0.25% SDS at 65° C. for 15 minutes, followed by a wash at 65° C. for a half hour to any one of:
the nucleotide sequence of SEQ ID NO:
70 or the nucleotide sequence of SEQ ID NO:
71, and
(b) at least a second nucleotide sequence comprising a nucleotide sequence that hybridizes under conditions comprising 0.5×SSC and 0.25% SDS at 65° C. for 15 minutes, followed by a wash at 65° C. for a half hour to any one of:
the nucleotide sequence of
SEQ ID NO:
77 or the nucleotide sequence of SEQ ID NO:
78.

6 more elements

Dependencies are inferred from the claim text.

Plants Modified With Mini-Chromosomes

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Plants Modified With Mini-Chromosomes

Life of this patent

Abstract

Claims71