Novel HIV Targets
OwnerAmy Espeseth, Daria J. Hazuda +1inventors
InventorsAmy Espeseth, Daria J. Hazuda, Min Xu
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Abstract
Using a method to measure the effect of downregulation of certain cellular proteins on HIV integration, host proteins implicated in HIV infection were identified. The identified proteins and encoding nucleic acids provide targets for inhibiting HIV infection and for evaluating the ability of compounds to inhibit HIV infection. Compounds inhibiting HIV infection include compounds targeting identified proteins and compounds targeting nucleic acids encoding the proteins.
Claims10
A method of identifying a host cellular protein involved in HIV infection comprising the step of measuring the ability of a siRNA library targeting different host cell factors to inhibit HIV infection, wherein measuring the ability of a siRNA library to inhibit HIV infection further comprises:
- transfecting human cells with the siRNA library targeting different cell factors;
- infecting the transfected cells with HIV;
- and
- assaying for viral infection to determine whether siRNA-mediated downregulation of host cell factors inhibits HIV infection.
(canceled)
An assay for identifying a compound as an HIV inhibitor comprising the steps of:
- identifying a compound that downregulates or otherwise inhibits the activity or expression of a target protein that is a component of a DNA repair pathway of a human cell;
- and
- determining the ability of said compound to inhibit HIV.
(canceled)
A method of treating HIV infection comprising decreasing the expression or activity of DNA repair pathway component selected from the group consisting of PMS2L1;
- POLI;
- TNP1;
- POLL;
- CENPF;
- MSH6;
- NEIL2;
- BTG2;
- DDB2;
- DCLRE1b;
- RTEL1;
- RAD51C;
- POLE;
- SMC6L1;
- APEX1;
- TAF2;
- OGG1;
- RUVBL2;
- RECQL4;
- TOP2A;
- ERCC3;
- RPA2;
- HMG4L;
- RBBP8;
- MLH1;
- MUS81;
- MSH4;
- IGF1R;
- RAD23B;
- ANKRD17;
- NTHL1;
- POLH;
- WDR33;
- DCLRE1A;
- and PMS1 in a patient in need thereof.
Dependencies are inferred from the claim text.