Transgenically preventing establishment and spread of transgenic algae in natural ecosystems
AssigneeTransAlgae Ltd
InventorsJonathan Gressel, Shai Ufaz, Ofra Shen, Doron Eisenstadt
Life of this patent
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Abstract
Genetic mechanisms for mitigating the effects of introgression of a genetically engineered genetic trait of cultivated algae or cyanobacteria to its wild type or to an undesirable, interbreeding related species. as well as preventing the establishment of the transgenic algae or cyanobacteria in natural ecosystems.
Claims42
A method to mitigate effects of release of at least one advantageous genetically engineered trait of an alga or a cyanobacterium into natural ecosystems, said method comprising a step of transforming an alga or a cyanobacterium to express said at least one advantageous genetically engineered trait, and at least one mitigating genetic trait, wherein:
- said at least one advantageous genetically engineered trait is encoded by at least one advantageous gene, and said at least one advantageous gene being operably linked with promoter sequences, and
- said at least one mitigating genetic trait is encoded by at least one mitigating gene, said at least one mitigating gene being optionally operably linked with promoter sequences;
- and
- said at least one advantageous gene and said at least one mitigating gene being introduced into the alga or cyanobacterium in tandem, whereby encoding sequences of the advantageous and mitigating genes remain genetically linked in the transgenic alga or cyanobacterium;
- and
- said at least one mitigating gene further being desirable in or neutral to the transgenic alga or cyanobacterium when cultivated but rendering the transgenic alga or cyanobacterium incapable of establishing itself or its introgressed offspring in natural ecosystems.
A genetic construct for mitigating effects of release of a genetically engineered genetic trait of an algae or cyanobacteria in to natural ecosystems, said construct comprising a first polynucleotide sequence encoding a desirable genetic trait and a second polynucleotide sequence encoding a mitigating genetic trait, wherein the first and the second polynucleotide sequences are covalently linked.
A genetic construct for mitigating effects of release of a genetically engineered genetic trait of an alga or cyanobacterium in to natural ecosystems, said construct comprising a first cassette and a second cassette, said first cassette comprising a first and a second polynucleotide sequences operably linked to a first and a second promoter sequences and encoding a first and a second desirable genetic trait,
- said second cassette comprising a polynucleotide sequence encoding a mitigating trait, said polynucleotide sequence being operably linked to a promoter sequence;
- and said first and second cassettes being covalently linked.
A method to mitigate effects of release of at least one advantageous genetically engineered trait of an alga or a cyanobacterium into natural ecosystems, said method comprising steps of:
- a. selecting a mutated alga or a cyanobacterium to express said at least one mitigating trait, and
- b. transforming the mutated alga or cyanobacterium with said at least one advantageous trait, wherein said at least one advantageous genetically engineered trait is encoded by at least one advantageous gene, and said at least one advantageous gene being operably linked with promoter sequences, and said at least one mitigating trait being desirable in or neutral to the transformed alga or cyanobacterium when cultivated but rendering the alga or cyanobacterium incapable of establishing itself or its introgressed offspring in natural ecosystems.
A method to mitigate effects of release of at least one advantageous genetically engineered trait of an asexual, or non-conjugating alga or a cyanobacterium into natural ecosystems, said method comprising a step of transforming an alga or a cyanobacterium to express said at least one
- advantageous genetically engineered trait, and at least one mitigating genetic trait, wherein:
- said at least one advantageous genetically engineered trait is encoded by at least one advantageous gene, and said at least one advantageous gene being operably linked with promoter sequences, and
- said at least one mitigating genetic trait is encoded by at least one mitigating gene, said at least one mitigating gene being optionally operably linked with promoter sequences;
- and
- said at least one mitigating gene further being desirable in or neutral to the transgenic alga or cyanobacterium when cultivated but rendering the transgenic alga or cyanobacterium incapable to compete in natural ecosystems.
Dependencies are inferred from the claim text.