Method of enzymatically synthesizing 3' -phosphoadenosine-5' -phosphosulfate

A DNA fragment having a nucleotide sequence of SEQ ID NO:

• 1, wherein one to several nucleotides are deleted, substituted, inserted, or added, which encodes an enzyme exhibiting ATPS activity.

A DNA fragment having a nucleotide sequence of SEQ ID NO:

• 2, which encodes Geobacillus stearothermophilus-derived adenosine 5′-phosphosulfate kinase (APSK).

A DNA fragment having a nucleotide sequence of SEQ ID NO:

• 2, wherein one to several nucleotides are deleted, substituted, inserted, or added, which encodes an enzyme exhibiting APSK activity.

A DNA fragment comprising a polynucleotide which is formed of nucleotides 101 to 1,171 in a nucleotide sequence of SEQ ID NO:

• 9, and which encodes polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), wherein a nucleotide sequence corresponding to at least 72 N-terminal amino acid residues is deleted.

An adenosine 5′-triphosphate (ATP) supply/regeneration system comprising adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and polyphosphate:AMP phosphotransferase (PAP).

An adenosine 5′-triphosphate (ATP) supply/regeneration system comprising adenosine 5′-monophosphate (AMP), polyphosphate, polyphosphate-driven nucleoside 5′-diphosphate kinase (PNDK), and adenylate kinase (ADK).