Transgenic plants with enhanced drought-resistance and method for producing the plants
AssigneeD-Helix
InventorsZhizhong Gong, XuHui Hong
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Abstract
Mutation of ELO2 is newly demonstrated to result in plants with increased sensitivity to abscisic acid (ABA).
Claims32
A method of enhancing abscisic acid sensitivity in a plant, the method comprising introducing an recombinant expression cassette into a plant, wherein the expression cassette comprises a promoter operably linked to a polynucleotide comprising at least 20 contiguous nucleotides complementary or identical to a contiguous sequence in a cDNA encoding an endogenous polypeptide at least 50% identical to SEQ ID NO:2, SEQ ID NO:4 or SEQ ID NO:6 in the plant, wherein the promoter is heterologous to the polynucleotide, thereby reducing expression of the polypeptide in the plant, wherein the plant has increased abscisic acid sensitivity compared to an otherwise identical plant lacking the expression cassette.
A recombinant expression cassette comprising a promoter operably linked to a polynucleotide comprising at least contiguous 20 nucleotides complementary or identical to a contiguous sequence in a cDNA encoding an endogenous polypeptide at least 50% identical to SEQ ID NO:2, SEQ ID NO:4 or SEQ ID NO:6 in the plant, wherein the promoter is heterologous to the polynucleotide, and wherein introduction of the expression cassette into a plant results in enhanced abscisic acid sensitivity in the plant compared to an otherwise identical plant lacking the expression cassette.
A transgenic plant comprising a recombinant expression cassette, the recombinant expression cassette comprising a promoter operably linked to a polynucleotide comprising at least 20 contiguous nucleotides complementary or identical to a contiguous sequence in a cDNA encoding an endogenous polypeptide at least 50% identical to SEQ ID NO:2, SEQ ID NO:4 or SEQ ID NO:6 in the plant, wherein the promoter is heterologous to the polynucleotide, and wherein the plant has enhanced abscisic acid sensitivity compared to an otherwise identical plant lacking the expression cassette.
Dependencies are inferred from the claim text.