Methods for performing direct enzymatic reactions involving nucleic acid molecules
AssigneeBioQuest
InventorsYoung Geun Yang, Jong Yeol Kim, Suhng Wook Kim, Sung Gwan Lee
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Abstract
Methods for performing a direct enzymatic reaction involving a nucleic acid molecule include performing the enzymatic reaction directly using a biological specimen in a reaction mixture containing a zwitterionic buffer and/or a non-reducing carbohydrate to prevent the biological specimen from inhibiting the enzymatic reaction, in which a nucleic acid molecule present in the biological specimen is not purified before the enzymatic reaction, and obtaining a product from the enzymatic reaction.
Claims34
A method for performing a direct enzymatic reaction involving a nucleic acid molecule, which comprises the steps of:
- (a) performing the enzymatic reaction directly using a biological specimen in a reaction mixture containing a zwitterionic buffer and/or a non-reducing carbohydrate to prevent the biological specimen from inhibiting the enzymatic reaction, wherein a nucleic acid molecule present in the biological specimen is not purified before the enzymatic reaction;
- and
- (b) obtaining a product from the enzymatic reaction.
A kit for performing a direct enzymatic reaction involving a nucleic acid molecule and directly using a biological specimen, comprising:
- a reaction mixture for the enzymatic reaction containing a zwitterionic buffer and/or a non-reducing carbohydrate to prevent the biological specimen from inhibiting the enzymatic reaction,
- wherein a nucleic acid molecule present in the biological specimen is not required to be purified before the enzymatic reaction.
Dependencies are inferred from the claim text.