Loading patent…
AssigneePharma Mar, S.A.
InventorsAngelina Ramos Castro, Alfredo Fernandez Brana, Felipe Lombo Brugos, Carmen Mendez Fernandez, Jose Antonio Salas Fernandez, Ana Velasco Iglesias, Carmen Schleissner Sanchez
Estimating the term…
The invention relates to genes involved in the biosynthesis of thiocoraline and to the heterologous production of same. According to the invention, the cluster of genes responsible for the biosynthesis of thiocoraline was identified and cloned. Said cluster of genes can be used in the heterologous production of thiocoraline which has an antitumor and antibacterial activity.
An isolated nucleic acid molecule comprising a nucleotide sequence encoding at least one biosynthetic thiocoraline production pathway protein, or a biologically active fragment thereof.
A host cell transformed or transfected with a vector of the invention.
A protein encoded by the nucleic acid molecule of the invention.
A method for producing thiocoraline which comprises growing, under suitable conditions for producing said compound, a thiocoraline-producing organism in which the number of copies of genes encoding proteins involved in the biosynthesis of thiocoraline has been increased, and, if desired, isolating thiocoraline.
A method for producing thiocoraline which comprises growing, under suitable conditions for producing said compound, a thiocoraline-producing organism in which the expression of the genes encoding the proteins responsible for the biosynthesis of thiocoraline has been modulated by means of manipulating or substituting one or more genes encoding proteins involved in the biosynthesis of thiocoraline or by means of manipulating the sequences responsible for regulating the expression of said genes, and, if desired, isolating thiocoraline.
A process, based on the use of genes responsible for the biosynthesis of thiocoraline from Micromonospora sp. ML1, for the production of said compound in another actinomycete, comprising:
Dependencies are inferred from the claim text.