Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
AssigneeWashington State University Research Foundation
InventorsJohn A. Browse, James G. Wallis, Jennifer L. Watts
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Abstract
The amino acid and nucleic acid sequences of a Δ5-desaturase enzyme and a Δ8-desaturase enzyme are disclosed. The nucleic acid sequences can be used to design recombinant DNA constructs and vectors. These vectors can then be used to transform various organisms, including for example, plants and yeast. The transformed organisms will then produce polyunsaturated fatty acids. The amino acid sequences are useful for generating enzyme-specific antibodies that are useful for identifying the desaturases.
Claims19
A method for creating a double bond between two carbons in a fatty acid, comprising:
- contacting a fatty acid with at least one purified desaturase, wherein the at least one purified desaturase is encoded by a nucleic acid sequence that hybridizes under high-stringency conditions to a nucleotide sequence with 95% sequence identity to SEQ ID NO:
- 3;
- and
- allowing the desaturase to create a double bond between two carbons.
Dependencies are inferred from the claim text.