Database for analyzing gene function and method of analyzing gene function by DSPA

A method of analyzing functions of a function-unknown gene (gx), which comprises:

• (a) measuring the viabilities, against a plural number of drugs (D1, D2, D3, . . . Dn) at various concentration, respectively, of transformed eukaryotic cells overexpressing a plural number of function-known genes (g1, g2, g3, . . . gn) and their parental cell lines;
• (b) calculating the ratio of the concentration of the drug to inhibit the viability of the transformed cell to an extent of 40% (IC40 value) to the IC40 value of the parental cell line;
• (c) calculating logarithmic values of the ratios of the above (b) for the function known genes (g1, g2, g3, . . . gn);
• (d) calculating a correlation coefficient among the function-known genes (g1, g2, g3, gn) for the logarithmic values of the above (c);
• (e) measuring the ID40 value for each drug from the viabilities at various concentration of a plural number of drugs (D1, D2, D3, . . . Dn) for transformed eukaryotic cells overexpressing the unknown gene (gx);
• (f) calculating the correlation coefficient between the unknown gene (gx) and known genes (g1, g2, g3, . . . gn) from the IC40 values of the above (e) by the same method of above (d);
• and
• (g) determining that the function of the known gene showing a significant correlation coefficient to the unknown gene (gx) is related to the function of the unknown gene (gx).