US 2009/0023594 A1 · REAGENTS FOR LABELLING NUCLEIC ACIDS AND USES THEREOF

Abstract

The present invention relates to labelling kits containing novel non-natural nucleotide monomers and to methods of making and using such compounds. The invention further relates to a method of detecting the presence of a nucleic acid, e.g., RNA, of interest in a sample, the method having the following steps: providing the sample; ligating a nucleic acid of interest with a labelling reagent according to the instant invention; providing a nucleic acid array having probes directed to the nucleic acid of interest; hybridizing the labelled nucleic acid fragments to said nucleic acid array; and determining the extent of hybridization to said probes to determine the presence of the nucleic acid of interest.

Claims31

Dependent claims

A method of detecting the presence of a nucleic acid of interest, said method comprising the following steps:

(a) providing a sample comprising nucleic acid that may or may not have said nucleic acid of interest;
(b) ligating said nucleic acid in said sample to a labelling reagent having the formula
or an ion thereof,
wherein B is a nucleobase;
R2 is a functional group that permits attachment to a 3′ OH group of said nucleic acid in said sample;
L is a linker group;
and R1 is a detectable moiety, to provide labelled nucleic acids;
(c) providing a collection of detection probes directed to said nucleic acid of interest;
(d) contacting the labelled nucleic acids with said collection under hybridizing conditions;
and
(e) determining the extent of hybridization of said labelled nucleic acids to said detection probes to determine the presence of said nucleic acid of interest.

8 more elements

Dependencies are inferred from the claim text.

Reagents for labelling nucleic acids and uses thereof

Life of this patent

Reagents for labelling nucleic acids and uses thereof

Life of this patent

Abstract

Claims31