Reagent, Medium, and Method for Detection of Alicyclobacilus Acidoterrestris
AssigneeMicrobio Co., Ltd.
InventorsHiroyuki Ogawa, Mitsuyoshi Miyashita
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Abstract
To supply the reagent, medium, and method for detection of Alicyclobacilus acidoterrestris, with which acidoterrestris can be rapidly detected and easily distinguished from acidocaldarius. After a sample including fruit juice is added to the medium for detection of Alicyclobacilus acidoterrestris containing chlor-2,3,5-triphenyl-2H-tetrazolium, acidoterrestris bacteria are detected based upon color change to red of the colony on the medium, which is kept at the temperature of 40 to 50° C.
Claims4
Reagent for detection of Alicyclobacilus acidoterrestris characterized by consisting of chlor-2,3,5-triphenyl-2H-tetrazolium.
Medium for detection of Alicyclobacilus acidoterrestris characterized by containing chlor-2,3,5-triphenyl-2H-tetrazolium.
Method for detection of Alicyclobacilus acidoterrestris characterized by detection of the above bacteria based upon color change of the colony on the above medium, which are kept under the conditions of culture of Alicyclobacilus acidoterrestris after a sample is added to the media for detection of Alicyclobacilus acidoterrestris containing chlor-2,3,5-triphenyl-2H-tetrazolium.
Method for detection of Alicyclobacilus acidoterrestris characterized by detection of the above bacteria based upon becoming red of the colony on the above media, which are kept at 40 to 50° C. after a sample containing fruit juice is added to the medium for detection of Alicyclobacilus acidoterrestris containing chlor-2,3,5-triphenyl-2H-tetrazolium.
Dependencies are inferred from the claim text.