Method for determining specific groups constituting heparins or low molecular weight heparins
AssigneeAventis Pharma S.A.
InventorsChristian Viskov, Pierre Mourier
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Abstract
The invention provides methods for analysing heparins, low-molecular-weight heparins, ultralow-molecular-weight heparins, and oligosaccharides by high performance liquid chromatography a stationary phase dynamically coated with a quaternary ammonium salt. The methods of the invention may be used to analyse samples without pre-treatment or to analyse samples that have been partially or exhaustively depolymerised and, optionally, reduced. Specific saccharides can be detected.
Claims34
A method of assaying a sample chosen from heparin, low-molecular-weight heparin, ultra low molecular weight heparin, and oligosaccharides, wherein the sample is analysed using a reversed phase column coated with a quaternary ammonium salt for chromatographic separation and analysis of a complex mixture of oligosaccharides.
A method of assaying a sample chosen from heparins, low-molecular-weight heparins, ultra low molecular weight heparins, and oligosaccharides comprising:
- (a) depolymerizing the sample by enzymatic depolymerization
- (b) reducing the depolymerized sample;
- and
- (c) assaying the sample of step (a) and/or step (b) by CTA-SAX chromatography.
Dependencies are inferred from the claim text.